DOWNLOAD Test Reports
DDRC SRL Healthcare Packages

DDRC SRL Clinical Test

Under Your Tests...Empower Your Health

Ancampopr3-antibodies



Why Get Tested

To help detect aid in the diagnosis of and sometimes monitor certain forms of the autoimmune disorder systemic vasculitis inflammation of blood vessels To help distinguish between Crohn disease CD and ulcerative colitis UC the two most common types of inflammatory bowel disease IBD as an adjunct to other IBD testing

When To Get Tested

When you have symptoms such as fever muscle aches and weight loss that your health practitioner thinks may be due to a vascular autoimmune disorder sometimes to monitor response to therapy When you have symptoms such as persistent or intermittent diarrhea and abdominal pain that your health practitioner suspects may be due to an IBD when your health practitioner wants to distinguish between CD and UC

Sample Required

A blood sample drawn from a vein in your arm

Test Preparation Needed

None




Common Questions


Tests for antineutrophil cytoplasmic antibodies ANCA may be used to Help detect and diagnose certain forms of autoimmune vasculitis including granulomatosis with polyangiitis Wegener granulomatosis microscopic polyangiitis and eosinophilic granulomatosis with polyangiitis Churg Strauss syndrome Sometimes this test may also be used to monitor treatment and or detect a relapse of these conditions For more detailed information on these conditions see the article on Vasculitis Help distinguish between ulcerative colitis UC and Crohn disease CD two common types of inflammatory bowel disease IBD ANCA are autoantibodies produced by a person s immune system that mistakenly attack proteins within the person s neutrophils white blood cell types The most common subsets of ANCA are those that target the proteins myeloperoxidase MPO and proteinase 3 PR3 Two types of tests may be used Most often ANCA tests are performed using indirect immunofluorescence microscopy IFA Serum samples are mixed with neutrophils to allow autoantibodies that may be present to react with the cells The sample is put on a slide and treated with a fluorescent stain The slide is then examined under a microscope and the resulting pattern noted The cytoplasmic pattern cANCA is associated with PR3 antibodies and the perinuclear pattern pANCA is associated with MPO antibodies Another possible pattern is atypical ANCA Myeloperoxidase antibodies and proteinase 3 PR3 antibodies may be individually and specifically tested using an immunoassay method Some laboratories will perform all three tests ANCA MPO and PR3 as a panel while others will perform MPO and PR3 only if an initial ANCA test is positive Additional tests that may be performed to aid in diagnosis include erythrocyte sedimentation rate ESR and or C-reactive protein CRP to check for inflammation complete blood count CBC to measure and evaluate white and red blood cells and urinalysis blood urea nitrogen BUN and creatinine to evaluate kidney function For some patients viral studies for hepatitis or cytomegalovirus may be ordered

Vasculitis An ANCA test and or tests for MPO and PR3 are ordered when a person has signs and symptoms that suggest systemic autoimmune vasculitis Early in the disease symptoms may be vague or nonspecific such as fever fatigue weight loss muscle and or joint aches and night sweats As the disease progresses damage to blood vessels throughout the body may cause signs and symptoms associated with complications involving various tissues and organs A few examples include Eyes red itchy eyes or pink eye conjunctivitis problems with sight blurry vision loss of vision Ears hearing loss Nose runny nose or other upper respiratory symptoms that do not go away Skin rashes and or granulomas Lungs cough and or difficulty breathing Kidneys protein in the urine proteinuria Testing may also be performed periodically to monitor a person who has been diagnosed with an autoimmune vasculitis Inflammatory Bowel Disease An ANCA test may be ordered with a test for anti-Saccharomyces cerevisiae antibodies ASCA when a person has signs and symptoms that suggest inflammatory bowel disease and the health practitioner is attempting to distinguish between Crohn disease and ulcerative colitis Symptoms of an IBD may include Abdominal pain and cramps Diarrhea Rectal bleeding Fever Fatigue In some people joint skin bone and organ-related symptoms Children may also have delayed development and growth retardation

Results of ANCA tests must be interpreted carefully taking several factors into account A health practitioner will consider clinical signs and symptoms in addition to results of the laboratory tests and other types of tests such as imaging studies Vasculitis Positive ANCA PR3 and or MPO tests help to support a diagnosis of systemic autoimmune vasculitis and to distinguish between different types However to confirm a diagnosis a biopsy of an affected site is often required Negative ANCA tests results mean it is unlikely that a person s symptoms are due to an autoimmune vasculitis For a positive result on the indirect immunofluorescence microscopy method several different ANCA patterns may be seen Perinuclear pANCA most of the fluorescence occurs near the nucleus About 90 of samples with a pANCA pattern will have MPO antibodies Cytoplasmic cANCA the fluorescence occurs throughout the cytoplasm of the cell About 85 of samples with a cANCA pattern will have PR3 antibodies Negative ANCA very little or no fluorescence If an ANCA test result is positive then an additional test is performed to determine the amount of antibody present This is called a titer To determine the titer a serum sample is diluted in steps and each dilution is tested for the presence of the antibody The greatest dilution at which the antibody can be detected is the titer For example if a serum tests positive after being diluted 64-fold the titer is 1 64 The higher the titer the more antibody is present in the blood ANCA levels can change over time and may sometimes be used in a general way to monitor disease activity and or response to therapy however titer levels may be inconsistent in some patients poorly reflecting remission relapse status In addition tests that are specific for antibodies to MPO and PR3 may be performed A positive test for PR3 antibodies and a positive cANCA or pANCA are seen in more than 80 of patients with active granulomatosis with polyangiitis Wegener granulomatosis A positive test for MPO antibodies and a positive pANCA are consistent with microscopic polyangitis glomerulonephritis eosinophilic granulomatosis with polyangiitis Churg Strauss syndrome and Goodpasture syndrome MPO and pANCA may also be present in other autoimmune disorders such as systemic lupus erythematosus rheumatoid arthritis and Sj gren syndrome The following table shows results that may be seen in some vasculitis conditions Condition of patients with cANCA pattern PR3 antibodies of patients with pANCA pattern MPO antibodies Granulomatosis with polyangiitis Wegener granulomatosis 90 in active disease 60-70 no active disease Less than 10 Microscopic polyangiitis 30 60 Eosinophilic granulomatosis with polyangiitis Churg Strauss syndrome Rare 50-80 Polyarteritis nodosa Rare Rare Inflammatory Bowel Disease ANCA testing can be useful in evaluating patients with symptoms of an inflammatory bowel disease IBD If atypical ANCA is positive and ASCA anti-Saccharomyces cerevisiae antibodies is negative then it is likely that the person has ulcerative colitis UC If atypical ANCA is negative and ASCA is positive then it is likely that the person has Crohn disease CD A person who is negative for ANCA and or ASCA may still have UC CD or another IBD

In most cases a biopsy of an affected blood vessel is necessary to confirm a diagnosis of autoimmune vasculitis Since the symptoms associated with vasculitis and inflammatory bowel disease may be seen with a number of conditions other tests are frequently performed prior to or along with ANCA testing to rule out other causes for the symptoms

Levels may fluctuate but once a person has developed an autoantibody that person will continue to have it

ANCA testing requires specialized equipment and careful interpretation by trained professionals Your sample will need to be sent to a clinical laboratory that performs these tests

ANCA testing is only necessary when a person has symptoms that suggest systemic vasculitis or inflammatory bowel disease IBD Most people will never need to have an ANCA test performed

Yes These conditions include rheumatoid arthritis systemic lupus erythematosus SLE pulmonary conditions autoimmune hepatitis use of certain drugs and infections involving the heart endocarditis or the respiratory system

There are many autoimmune disorders that have been identified see the article on Vasculitis or the Related Pages tab for more on these They are organized with some overlap by the size of the blood vessels that they affect small medium or large